MSDOS.SYS an important system file on MS-DOS and Windows 9x systems. It is run after IO.SYS. In MS-DOS, it contains the core operating system code, the kernel. The Windows Setup program creates a file called Msdos.sys in the root folder and sets the file with the Read-Only, System, and Hidden attributes. Unlike the Msdos.sys file in MS-DOS, this file is a text file. It contains a [Paths] section that lists the locations for other Windows files (such as the registry) and an [Options] section that you can use to personalize the boot process.
By default the file is located in the root directory of the bootable drive/partition (normally C:\) and has the hidden, read-only and system file attributes set.
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